Fig. 6.

Less bladder myeloperoxidase (MPO) activity and edema in meprin αKO vs. WT after transurethral administration of LPS. LPS (20 μg) was instilled into the bladder via catheter. Mice were necropsied at multiple time points, and leukocyte infiltration was measured by MPO assay. A: bladders from WT mice had significantly more MPO U/g bladder tissue vs. meprin αKO. *P = 0.01 at 24 h. **P < 0.015 at 48 h. MPO activity was low in saline controls (0.3 ± 0.18 MPO U/g). B: bladder-to-body weight ratios were higher in WT vs. αKO, indicating more bladder edema. *P = 0.007 at 3 h. **P < 0.01 at 6 h. ***P < 0.005 at 12 h. #P < 0.02 at 24 h. The data are an average of 2 independent experiments, with n = 8–12 mice per genotype, per time point. Dashed lines indicate average value for saline-treated control animals.