Table 1.
Primer sequences for real-time PCR
| Gene | Sequence | Size, bp |
|---|---|---|
| ICAM-1 | Fwd: GCCCGGAGGATCACAAACGAC | 186 |
| Rev: CCTGGGGCTGGCATGTAAGAGT | ||
| VCAM-1 | Fwd: GGGGGCCAAGTCCGTTCTGA | 158 |
| Rev: GGGGGCCACTGAATTGAATCTC | ||
| E-selectin | Fwd: CCCTGACCATGGAAGCCTGAAC | 163 |
| Rev: ATGGCAGGCAGGAGCAGGTG | ||
| P-selectin | Fwd: CGGCGGAGGCTGAGAACTG | 191 |
| Rev: GCGCTCCCCTTGGCTGTT | ||
| Collagen IVα5 | Fwd: AAGGGGGAGCCAGGCAGTATAA | 118 |
| Rev: TTGGCCCTGATACACCCTTGG | ||
| TGF-β1 | Fwd: ATACGCCTGAGTGGCTGTCT | 153 |
| Rev: TGGGACTGATCCCATTGATT | ||
| VEGF-A | Fwd: TGCGGATCAAACCTCACCAAA | 147 |
| Rev: TGCGCTTTCGTTTTTGACCC | ||
| VEGF-C | Fwd: CCACAGTGTCAGGCAGCTAA | 157 |
| Rev: ACTCCTTGTTGGGTCCACAG | ||
| VEGFR1 | Fwd: TGCAAGCGGGCCAGACTC | 163 |
| Rev: CGCCATGTTCAAGGTCAAGGT | ||
| VEGFR2 | Fwd: GCCGGCATGGTCTTCTGTGAG | 109 |
| Rev: GGGGGCTCAGGACCACATCATA | ||
| Angiopoietin 1 | Fwd: TGCCGTGAGAGTGCGACAGA | 181 |
| Rev: GGGCCATCTCCGACTTCATATT | ||
| Angiopoietin 2 | Fwd: GGAGGAGGGTGGACGGTCATC | 150 |
| Rev: GCGATGCCCACTGGTCAGCT | ||
| Tie-2 | Fwd: CCACACACATTTGGCAGGACCT | 245 |
| Rev: AGCCCTTGCCATCCTTGAGAGC | ||
| MCP-1 | Fwd: AGCCCAGAAACCAGCCAACTC | 196 |
| Rev: GCCGACTCATTGGGATCATCTT | ||
| IL-2 | Fwd: TGCAGCGTGTGTTGGATTTGAC | 144 |
| Rev: TTGCTGGCTCATCATCGAATTG | ||
| IFN-γ | Fwd: AACCAGGCCATCAGCAACAACA | 214 |
| Rev: ACCGACTCCTTTTCCGCTTCCT | ||
| GAPDH | Fwd: GCCAAAAGGGTCATCATCTC | 229 |
| Rev: GGCCATCCACAGTCTTCT |
ICAM, intercellular adhesion molecule; VCAM, vascular cell adhesion molecule; TGF, transforming growth factor; VEGF, vascular endothelial growth factor; VEGFR, VEGF receptor; MCP, monocyte chemoattractant protein; IFN, interferon; Fwd, forward; Rev, reverse.