Fig. 1.

PUFAs from triglyceride-rich lipoprotein (TGRL) incubated with or without lipoprotein lipase (LpL). Human postprandial VLDL (A), chylomicron (CM) (B), or TGRL (C) [2.5 mg triglyceride (TG) for all treatments] with or without LpL were used for lipid extraction and oxidized lipids measurement. For LpL-dependent lipolysis, LpL was preincubated with CM, VLDL, or TGRL for 30 min at 37°C, and the reaction was stopped by transferring the tubes on ice prior to sample extraction and FA analysis. The concentration of linoleic acid (LA) and arachidonic acid (AA), as well as the n-3 FAs α-linoleic acid (ALA), eicosapentenoic acid (EPA), and docosahexaenoic acid (DHA) in VLDL, CM, and TGRL increased with LpL treatment. ^a Lipids were pseudo-quantified and adjusted by relative response factors to estimate their concentrations and allow consistent graphical representation of these data. Data are mean ± SEM (n = 3).