Fig. 2.

Oxidized lipids from VLDL incubated with or without LpL. Concentrations of oxylipids were increased by LpL treatment. Human postprandial VLDLs (2.5 mg TG) with or without LpL were used for lipid extraction and oxidized lipids measurement. For LpL-dependent VLDL lipolysis, LpL was preincubated with VLDL for 30 min at 37°C, and the reaction was stopped by transferring the tubes on ice prior to sample extraction. The LA oxidation products (A) epoxy octadecenoic acid (EpOME), dihydroxy octadecanoic acid (DHOME), hydroxy ocatadecadienoic acid (HODE), and oxo octadecanoic acid (oxo-ODE), as well as the AA oxidation products (B) epoxy eicosatrienoic acid (EET), dihydroxy eicosatrienoic acid (DHET), hydroxy eicosatetraenoic acid (HETE), and oxo eicosatetraenoic acid (oxo-ETE) were determined. Data are mean ± SEM (n = 3).