Fig. 4.
RSV blocks 10,12 CLA-mediated insulin resistance. Cultures of newly differentiated human adipocytes were serum starved for ∼24 h and then treated for either 48 h (A) or 24 h (B) with BSA vehicle or 50 μM 10,12 CLA in the absence (−) or presence (+) of 50 μM RSV (R). A: Insulin-stimulated glucose uptake using 2-[3H]deoxyglucose was measured after a 90 min incubation in the presence of insulin. Means (±SE; n = 6) not sharing a lower-case letter differ significantly (P < 0.05). B: The mRNA levels of suppressor of SOCS-3, adiponectin (APM-1), and sirtuin 1 (SIRT-1) were measured by real-time qPCR. Means (±SE; n = 3) not sharing a lower-case letter differ significantly (P < 0.05) and are representative of at least two independent experiments.