TABLE 1.

Relative mRNA levels of eNOX1, eNOX2, NQO1, and NQO2 in human aortic endothelial cell (HAEC)

Gene	GAPDH	eNOX1	eNOX2	NQO1	NQO2
Ct value	13.83 ± 0.153	24.33 ± 0.141	23.73 ± 0.141	15.93 ± 0.153	21.0 ± 0.00
Relative mRNA copy numbera	1,000	0.69	1.05	233.94	6.98

^aValue means mRNA copy number per thousand copies of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA in HAEC. Copy numbers were calculated from Ct value of QRT-PCR. Each primer set showed ideal double expansion in PCR reactions as determined by standard reactions using known amounts of templates in the reactions. There was no formation of primer dimers, which was determined by melting dissociation curve. Values are mean ± SD of triplicate PCR reactions.