Fig. 3.

NR1 and NR1 splice variants’ expression in the thoracic T1–T3 dorsal root ganglia (DRGs). The relative changes in NR1 splice variant expression in cats receiving either acid or PBS was determined by semiquantitative RT-PCR amplification of RNA isolated from thoracic T3 DRGs. A: staining patterns (n = 3 cats/group) were NR1 splices with or without NH₂-terminal exon 5 (NR1-a/b), COOH-terminal exons 21 and 22 (NR1-1, NR1-2, NR1-3, NR1-4) in acid- and PBS-treated cats. B: intensity of expression for each gene product was normalized against rRNA expression. Values are expressed as means ± SE of 3 RT-PCR determinations (*P < 0.05, and **P < 0.001). MW; molecular weight.