Table 1.

RT-PCR primers for NR1 and NR1 splice variants

NR1 Amplicon Outside COOH-Terminal Exons		Exons	Product
NR1-1 forward	5′ GGCAGTAAACCAGGCCAATA 3′		184 bp
NR1-1 reverse	5′ GTGGGAGTGAAGTGGTCGTT 3′
NR1 splice variants
Common forward primer	5′ CCACGGGTGAGCTGTTCTTC 3′
NR1-1 reverse	5′ CCCGGTGCTCGTGTCTTTGG 3′	+21, +22	456 bp
NR1-2 reverse	5′ CCGGTGCTCTGCAGGTTCTTC 3′	−21, +22	355 bp
NR1-3 reverse	5′ TGGGATGGTACTGCGTGTCT 3′	+21, −22	471 bp
NR1-4 reverse	5′ GATGGTACTGCTGCAGGTTCTT 3′	−21, −22	357 bp
NR1 a/b forward	5′ CAGCGTCTGGTTTGAGATGA 3′	a: −5	a: 180 bp
NR1 a/b reverse	5′ AGCAGAGCCGTCACATTCTT 3′	b: +5	b: 243 bp
rRNAF1 forward	5′ TCAAGAACGAAGTCGGAGG 3′		498 bp
rRNAR1 reverse	5′ GGACATCTAAGGGCATCACA 3′
rRNAF2 forward	5′ GACCATAAACGATGCCGACT 3′		204 bp
rRNAR2 reverse	5′ GTGAGGTTTCCCGTGTTGAG 3′
Real-time PCR primers for NR1 and NR2A subunits
RNR1-1 forward	5′ CGAGAAGGAGAACATCAC 3′		103 bp
RNR1-1 reverse	5′ ATACTTGGAAGACATCAGC 3′
RNR2A forward	5′ GGCACGCTACAGAGACTC 3′		129 bp
RNR2A reverse	5′ GCACTCCTACGACAACATTC 3′