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RT-PCR results from representative experiments showing expression of mRNA for N-methyl-d-aspartate (NMDA) receptors NMDAR1, NMDAR2A, and NMDAR2B in carotid body (CB, left lane). RNA isolated from rat brain cerebral cortex was used as positive control (Brain, middle lane). Sterile water instead of cDNA (H2O, right lane) was used as PCR negative control. PCR product was not detected if reverse transcriptase was omitted from the reaction in tubes [Actin (−)].
