Table 2.
Primer and sequence for NMDA receptors and PSD-95
| Gene Product | GenBank Accession No. | Primer Sequence (5′-3′) | PCR Cycle No. | Annealing Temperature, °C |
|---|---|---|---|---|
| NMDAR1 | NM_017010 | F: 269-AGCACCATGCACCTGCTGACA-289 | 33 | 56 |
| R: 511-GAGGTCCTCACACACTGACAGG-490 | ||||
| NMDAR2A | M91561 | F: 3707-ACTCCACACTGCCCATGAAC-3726 | 34 | 53 |
| R: 4231-AGAGTTTGCTTGAGGGGACA-4212 | ||||
| NMDAR2B | NM_012573 | F: 3574-ACATGAAGTATCCAGTATGG-3593 | 33 | 56 |
| R: 4098-GTTCTGGTTGTAGCTGACAG-4079 | ||||
| NMDAR2C | U08259 | F: 1703-CGATGGCGTCTGGAATGG-1722 | 40 | 52 |
| R: 2316-CTGGCAAGAAAGATGACCGC-2297 | ||||
| NMDAR2D | NM_022797 | F: 2281-CGATGGCGTCTGGAATGG-2298 | 40 | 53 |
| R: 2745-CTGGCAAGAAAGATGACCGC-2726 | ||||
| PSD-95 | M96853 | F: 211-TTGCAGGTGAATGGAACAGAG-231 | 36 | 52 |
| R: 825-AGCATAGCTGTCACTCAGGTA-805 |
NMDA, N-methyl-d-aspartate; F, forward primer; R, reverse primer.