Pax7 levels in pectoralis muscle-derived myoblasts and muscle tissue are upregulated in response to TM (3H, 6H). Pectoralis muscle samples were removed in parallel from individual embryos or chicks from all groups (n = 4) at various days of age. On each day, samples derived from the control and TM groups were electrophoresed side by side on an SDS-polyacrylamide gel, and protein expression levels were evaluated by Western blot analysis. Bands were quantified by densitometry relative to α-tubulin, and results are means ± SE presented as fold induction relative to control. a,b,cData with different letters differ significantly within the same age group (P < 0.05).