Fig. 6.
EphrinB2 (FITC; E–H and Q–T) and EphB4 (FITC; A–D and M–P) pronounced expression is found in cells encompassing (white line) vascular structures (PECAM-1-Cy3; A–H and M–T) in day E13.5 (A–L) and day E15.5 (M–X) lungs. PECAM-1 (Cy3)-positive cells coexpress EphrinB2 (E–H and Q–T) and EphB4 (A–D and M–P). Epithelial and vascular coexpression of EphrinB2 and EphB4 is found at day E13.5 (I–L) and day E15.5 (U–X). EphrinB2 (Cy3) and EphB4 (FITC) are noted. FACS analysis indicates coexpression of EphrinB2 and EphB4 in PECAM-1-positive cells at day E13.5 (Y). By day E15.5 (Z), PECAM-1-positive cells predominately express the EphB4. Magnification, ×400–600.