Fig. 7.

cGKI protein levels in the soluble fraction of tissue homogenates from porcine PA with a quantitative immunoblotting method. Inset: representative immunoblot using known amounts of recombinant, purified cGKI to create a standard curve and 1 μg of homogenate from freshly prepared (0) and PA cultured for 24 h without (24) and with (24 NO) DETA-NO. cGKI levels are decreased in PA at 24 h and are further reduced at 24 h in the presence of DETA-NO. *Different from freshly prepared, #different from 24 h (P < 0.05). Data are means ± SE; n = 6.