Fig. 2.
Effect of increased pressure on exposure of the β1-integrin 9EG7 epitope. A: effect of functional blockade of β1- and β3-integrin on pressure-stimulated SW620 cell adhesion to 12 μg/ml fibronectin (n = 6). B: induction of the 9EG7 epitope on SW620 cells was assessed following exposure to ambient or 15-mmHg increased pressure for 30 min in the presence of DMSO, 1 mM MnCl2, or 1 μM cytochalasin D (n = 4). Representative histograms are shown (right) of typical 9EG7 staining following exposure to increased (red) or ambient (blue) pressure conditions. Solid gray peaks represent the unstained cell populations. C: effect of increasing pressures on 9EG7 induction (n = 3). MFI values of 9EG7-positive cells were determined by flow cytometric analysis. Data from individual experiments were normalized to respective IgG- or DMSO-treated ambient pressure controls and are graphically expressed as means ± SE. *P < 0.05 compared with ambient pressure control.