Table 1.

Immunodominant TCR repertoire of analyzed CTL lines generated by pp65 peptide pool mix and single pp65 (NLVP) peptide^a

Patient	AA Sequence of ID Clone	Vβ Family	Jβ Family	% of All Clones
pp65 peptide pool mixb
1	CASSLEQHTEAFF	14S1	1–1	62
2	CASSSQTGTIYGYTF	13S1	1–2	52
4a	CASSSRQGADTGELFF	6S4	2–2	25
4b	CASRGLSGLTEAFF	13S1	1–1	33
5	CASQGTAATNTGELFF	17S1	2–2	56
9	CASTWTGPDQPQHF	17S1	1–5	38
14a	CAANQREPYEQYF	13S1	2–7	48
14b	CASSAWTSGLNEQFF	17S1	2–1	33
19	CASSYQTGAAYGYTF	13S1	1–2	90
28a	CASKWGPANPEAFF	14S1	1–1	32
28b	CASKQGAGGNTEAFF	14S1	1–1	41
30	CASSPQTGVGYGYTF	13S1	1–2	70
Single pp65 (NLVP) peptide
1	CASSLEQHTEAFF		1–1	88
4a	CASSSRQGADTGELFF		2–2	35
4b	CASRGLSGLTEAFF		1–1	30
5	CASQGTAATNTGELFF		2–2	86
19	CASSYQTGAAYGYTF	13S1	1–2	79
28a	CASKWGPANPEAFF	14S1	1–1	36
28b	CASKQGAGGNTEAFF	14S1	1–1	38

^aThe results were obtained from 3 independently performed sequencing analyses for each cell line/CTL separation. The mean size/percentage of the immunodominant clone is shown.

^bCell lines obtained from patients 2, 19, and 30 showed the same Vβ and Jβ usage (italics), length of AA sequence, and central TCR motif (QTG, underline). For proving that the found TCR repertoire is not altered by in vitro expansion, TCR of pp65 peptide pool–specific cells obtained directly after their FACS separation were additionally analyzed in patients 1, 4, 5, 19, and 28. The found sequences of immunodominant clones were identical within the same patient.