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Normal TNF-α–primed neutrophils were stimulated as described already. Supernatants from these cells were incubated for 15 min with normal serum. This conditioned serum was used in different neutrophils for priming (45 min at 37°C), after which cells were activated with a mAb to PR3. ROS production was measured after 45 min of stimulation with αPR3, and data are means ± SEM (given as ratio to buffer-incubated normal serum [−], which was set at baseline to 100%). **P < 0.01.
