Figure 2.

Determination of PAR-1-mediated properties of variants of APC by calcium flux and EPCR binding. (A) APC induces calcium flux in HUVEC. Wt-APC, K193E, and L8W were used at 80 nM in the presence of 1.0 U/ml hirudin. The wt-APC peak was normalized to 100%. (B) Determination of the calcium flux in HUVECs at varying concentrations of wt-APC and the variants. The area under the curve was determined using FLIPR software (v2.12, Molecular Devices, Sunnyvale, California). Results are mean ± SEM, n = 8.