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. Author manuscript; available in PMC: 2009 Feb 6.
Published in final edited form as: Stem Cells. 2007 Jun 21;25(9):2235–2244. doi: 10.1634/stemcells.2007-0111

Figure 2. Immunohistochemistry performed on EBs after 14 days inside of fibrin scaffolds for mature cell markers including Tuj1 (neurons), O4 (oligodendrocytes) and GFAP (astrocytes).

Figure 2

A–C) In scaffold staining of EB culture when no growth factors were present. D–F) In scaffold staining of EB culture when 2 ng/mL of PDGF was present. G–I) In scaffold staining of EB culture when 10 ng/mL of Shh was present. J–L) In scaffold staining when 25 ng/mL of NT-3 was present. Scale bar for Tuj staining are 100 µm. Scale bars for O4 and GFAP staining is 50 µm.