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. Author manuscript; available in PMC: 2009 Oct 10.
Published in final edited form as: J Mol Biol. 2008 Jul 16;382(3):692–707. doi: 10.1016/j.jmb.2008.07.022

Fig. 4. SDS-PAGE analysis with non-reducing sample buffer of wild-type and mutated DsrC proteins purified under aerobic conditions in the absence of reducing agent.

Fig. 4

Lane 1, wt DsrC dimeric, Lane 2, DsrC-Cys100Ser dimeric, Lane 3, DsrC-Cys111Ser monomeric, Lane 4 DsrC-Cys100Ser/Cys111Ser monomeric (a) proteins as isolated (b) proteins pretreated with iodoacetamide leading to alkylation of free cysteine residues (c) proteins reduced with 2-mercaptoethanol after treatment with iodoacetamide