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. Author manuscript; available in PMC: 2009 Oct 10.
Published in final edited form as: J Mol Biol. 2008 Jul 16;382(3):692–707. doi: 10.1016/j.jmb.2008.07.022

Fig. 5. 1H-15N-HSQC NMR spectra of DsrC in oxidizing conditions.

Fig. 5

(a) Reduced DsrC. (b) Aged two weeks under air. (c) Oxidized by addition of ~20 mM H2O2 to reduced DsrC. (d) After 48 hrs in ~20 mM H2O2. In (b) and (d), non-uniform peak appearance suggests partially disordered structure, though some peaks remain unchanged. In (c) versus (a), slight upfield 15N shift of V112 peak indicates rapid Cys111-Cys111 disulfide bond formation.. Similarity of (b) and (d) show that the oxidized state is reached by air or H2O2 oxidation. Re-reduction of the oxidized state with DTT yields spectra indistinguishable from (a).