Fig. 5.
Deletion of the N- or C-terminus of MECP2 does not have any significant impact on its mobility. (A) Cells expressing EGFP-tagged MECP2e2 (WT), ΔN and ΔC were photobleached and their recovery curves were plotted, revealing essentially no differences in binding kinetics for the deleted proteins. (B) Curve fitting was used to determine t50 values±s.d. for each of the protein forms (21.3±5.4, 17.6±9.3 and 23.9±7.8 for WT, ΔN and ΔC, respectively). The t50 values of ΔN and ΔC are not significantly different from WT (P>0.05). The number of nuclei photobleached in each group is shown within the bars.