Skip to main content
. Author manuscript; available in PMC: 2009 Feb 6.
Published in final edited form as: Biochemistry. 2008 Dec 30;47(52):13878–13886. doi: 10.1021/bi801745u

FIGURE 4.

FIGURE 4

CW X-band EPR spectra of spin labeled cysteine MsbA mutants from the Walker B motif. Proteins were reconstituted into lipid membranes and spectra recorded in the absence of substrate (top), in the presence of ATP and EDTA (ATP) prior to hydrolysis, in the presence of MgATP followed by immediate addition of Vi (ADP/Vi) to trap the posthydrolysis state, and in the presence of MgATP (ADP) to allow hydrolysis to proceed. The arrow indicates the fast-motion component observed in many of the spectra shown.