Figure 8.

Thrombin generation in the plasma of lactadherin-deficient mice and control littermates. The reaction mixture consisted of plasma (80 μL) and the trigger solution (3 ng purified Russell viper venom in 40 μL), fluorogenic thrombin substrate Z-Gly-Gly-Arg-AMC (2.5 mM), and CaCl₂ (10 mM). The generation of thrombin was measured as a function of fluorescence in a fluorimeter over a period of 30 minutes. Thrombin was then calculated using an α₂-macroglobulin thrombin complex standard as described by Hemker et al.²⁷ Representative data are presented. Blood samples from 5 mice from each group (lactadherin^−/− and littermate controls [lactadherin^+/+]) were pooled for each experiment.