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Northern and Western analyses of PSA mRNA and protein and AR mRNA in ARCaP cells. ARCaP cells contain low levels of PSA mRNA and protein (A) and AR mRNA (B) as compared with LNCaP cells. Total cellular RNA used for Northern blots was as follows: PSA, 10 μg for LNCaP cells and 50 μg for ARCaP cells; AR, 20 μg each for LNCaP and ARCaP cells. Western blot analysis of PSA protein was performed by loading 25 and 50 μg of protein derived from LNCaP and ARCaP cells, respectively.
