Figure 1. Mena^INV Promotes Carcinoma Cell Motility In Vivo and Enhances Metastasis to the Lungs.

(A) Intravital imaging of tumors generated by injection of MTLn3 EGFP-Mena cells into rats. Scale bar, 20 μm. Green = EGFP-Mena; purple = collagen second harmonic.

(B) Intravital imaging of tumors generated by injection of MTLn3 EGFP-Mena cells into rats. Scale bar, 20 μm. Green = EGFP-Mena; purple = collagen second harmonic. Protrusion containing Mena is outlined in white.

(C) Table showing the quantification of motile cells in orthotopic breast tumors derived from injection of MTLn3 cells into SCID mice. *p < 0.002, **p < 0.04, unpaired t test with Welch’s correction for unequal variance.

(D) Average weight of primary tumors in xenograft SCID mice derived from injection of each cell type. Error bars indicate SEM.

(E) Spontaneous lung metastases quantified using multiphoton microscopy. Insert depicts picture of lung metastasis from an SCID mouse with a tumor derived from injection of MTLn3-EGFP-Mena^INV, taken using multiphoton microscopy. Green = MTLn3-EGFP-Mena^INV; purple = collagen second harmonic. Scale bar, 50 μm. Error bars indicate SEM. **p < 0.001; ***p < 0.00001.

(F) Spontaneous lung metastases quantified using H&E stained lung sections. Insert depicts H&E staining of lung section from MTLn3-EGFP-Mena^INV-derived xenograft. Dark purple = metastasis. Scale bar, 20 μm. Error bars indicate SEM. ***p < 0.001.