Skip to main content
. Author manuscript; available in PMC: 2009 Dec 1.
Published in final edited form as: Dev Cell. 2008 Dec;15(6):813–828. doi: 10.1016/j.devcel.2008.09.003

Figure 3. Expression of Mena or MenaINV Promotes Lamellipod Protrusion and In Vivo Invasion in Response to Low EGF Concentrations.

Figure 3

(A) EGF dose-response curve of EGFP- and EGFP-MenaINV-expressing MTLn3 cells. The area of the cells was determined 3 min after stimulation with the indicated EGF concentrations and normalized to the cell area before EGF treatment. Results represent duplicates; over 30 cells analyzed. Error bars indicate SEM.

(B) In vivo invasion assay: EGF dose-response curve of invasive cells collected from EGFP and EGFP-MenaINV tumors. Data normalized to average number of cells in needles containing 0.0 nM EGF for each type of xenograft. Error bars indicate SEM.

(C) Lamellipod protrusion after 0.5 nM EGF stimulation. Results represent triplicates; over 45 cells analyzed. Error bars indicate SEM. Magnification of the boxed area from the left graph is shown on the right.

(D) Lamellipod protrusion after 5 nM EGF stimulation. Results represent triplicates; over 40 cells analyzed. Error bars indicate SEM.