Bcl-2 coimmunoprecipitated with GRs and translocated into mitochondria after CORT treatment in a time- and dose-dependent manner. (A) Bcl-2 coimmunoprecipitates with GRs in cultured cortical neurons after 30-min treatment (lane1–3: IP with anti-GR antibody; lane 4: total protein from cortical neurons; lane 5: IP with anti-IGF antibody; lane 6: no antibody). (B) The formation of GR/Bcl-2 complexes was enhanced in cortical neurons after 30-min treatment with CORT (1 μM and 100 nM) (n = 6, n = 5–8, Student's t test P < 0.05). (C) Bcl-2 translocated into mitochondria after CORT treatment in cultured cortical neurons (n = 2–3, n = 8–14, Student's t test, **P < 0.01, *P < 0.05). Data are presented as mean ± SE.