Fig. 3.

Estrogen promotes the lung colonization of Tsc2-null ELT3 cells. (A) ELT3-luciferase cells were injected intravenously into ovariectomized female placebo (n = 3) and E₂-treated (n = 3) mice. Lung colonization was measured using bioluminescence at 1, 3, and 24 h after injection. Representative images are shown. (B) Total photon flux/second present in the chest regions in placebo (n = 3) and E₂-treated (n = 3) animals. *, P < 0.05, Student's t test. (C) Lungs were dissected 24 h postcell injection and bioluminescence was imaged in Petri dishes.