Figure 2.

A P18 derivative mutant, P18 157m, is creating a net-like sheath on Sf9 cell MTs. Cells infected for 3 days with a baculovirus recombinant producing P18 157m were prepared for electron microscopic observations as in ref. 15. A thin section of an infected cell (a) was gold-labeled with a P18 antibody (a1). After being treated with Taxol, infected cells were disrupted in AB buffer, negatively stained, and observed (b and c). P18 157m net-like structure and well-shaped MTs are indicated by large and small arrows, respectively. (d) An infected cell was trapped on a microscope grid and gold labeled with a P18 antibody. (Bars = 500, 250, 75, 50, and 400 nm in a, a1, b, c, and d, respectively.)