Figure 6. Neither LPA1 nor LPA2 are required for isolated SMC dedifferentiation.
Expression of SMC-specific myosin heavy chain in cultured SMCs after serum deprivation, or following re-exposure to either 10% FCS or 1 μM LPA for 24 hours (A). Rho activity was examined 10 min after exposure of cells to LPA (1 μM) by Rhotekin pulldown assay (B). Values represented results obtained from three separate experiments and are reported as a fold change from baseline in WT cells (mean ± SD). S1P = sphingosine-1-phosphate.