Figure 1.

Rng3 associates with polysomes. Extracts were fractionated using ultracentrifugation through sucrose gradients and Rng3-TAP was detected by Western blot. The top of the gradient is shown on the left. The position of the ribosomes was determined from the absorbance at 254 nm and confirmed by the distribution of the large subunit ribosomal proteins Rpl7 (data not shown). Ribosomes were stabilized by treatment of cells with cycloheximide, or disassembled by the addition of EDTA, RNase or puromycin to the extracts.