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. 2008 Dec 19;10(2):186–191. doi: 10.1038/embor.2008.228

Figure 3.

Figure 3

The Rng3–myosin messenger RNA association is sensitive to conditions that destabilize polysomes. (A) Western blots of samples from Rng3-TAP immunoprecipitation experiments from mock-treated (−) or EDTA-treated extracts (+). Here, 1.5% of the input and 5% of the IP were loaded. (B) Enrichment of myosin RNAs in Rng3-TAP IPs (normalized to the levels of the small nuclear RNA U3) from mock- (grey) or EDTA-treated extracts (white). Data from two independent experiments are shown. EX, whole-cell extract; IP, immunoprecipitate; SN, supernatant after immunoprecipitation; TAP, tandem affinity purification.