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. 2009 Jan 16;10(2):173–179. doi: 10.1038/embor.2008.238

Figure 1.

Figure 1

Ubiquilins protect against starvation-induced cell death. (A) HeLa cells expressing GFP-tagged UBQLN1 or UBQLN2 (or GFP for controls) were transferred to starvation medium (PBS) for the indicated time. Where specified, only serum was removed from the culture medium. Viability of transfected cells was assessed by PI staining and analysed by flow cytometry. The graph represents the mean of three experiments±s.e. (B) HeLa cells were depleted of UBQLN1 or UBQLN2 by siRNA transfection; depletion was verified by Western blot. Cells were starved for increasing periods of time, and their viability was analysed as in (A). The graph represents the mean of three experiments±s.e; *P<0.05 between control and UBQLN2-KD at 6 h (paired Student's t-test). GAPDH, glyceraldehyde-3-phosphate dehydrogenase; GFP, green fluorescent protein; KD, knockdown; PI, propidium iodide; siRNA, short interfering RNA; UBQLN, ubiquilin.