Figure 6.

LPS-binding capacity of different forms of mindin. Full-length mindin from HEK 293 cells (mMD), full-length mindin from E. coli (bMD), mindin FS domain alone from E. coli (bMD-FS) and human serum albumin (HSA) were coated on ELISA plate, which was washed and incubated with LPS from Salmonella typhosa (10 μg/ml) at room temperature for 1 h as described (He et al, 2004). Bound LPS was detected using mouse anti-LPS antibody. The error bars represent standard deviations of triplicate wells.