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A. Schematic representation of structure of each allele and positions of primers. Primers A and B are used to differentiate the conditional allele from WT, whereas primers C and D are used to differentiate the fx allele from fn. Primers B and C are used to identify the Cre-recombined allele.
B. Genotyping results of the conditional allele. Combinations of the primers and sizes of the amplicons are indicated.
