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. Author manuscript; available in PMC: 2009 Feb 8.
Published in final edited form as: Circulation. 2006 Jul 4;114(1 Suppl):I200–I205. doi: 10.1161/CIRCULATIONAHA.105.000240

Figure 5.

Figure 5

rhCTGF induces type I and III collagens in cultured VSMCs in a dose- and time-dependent fashion. VSMCs were maintained in serum-free conditions for 48 hours. In the presence of 50 μg/mL ascorbic acid and 0.2 mmol/L [l]proline, VSMCs were incubated with increasing doses of rhCTGF for 48 hours or at a concentration of 50 ng/mL for up to 48 hours. Unstimulated medium was used as a negative control (0 ng rhCTGF). Conditioned supernatants of cultured cells were collected for use in the Western blot analyses. rhCTGF induced type I collagen synthesis, as shown by the increased levels in the supernatant of treated VSMCs, in a dose- (A, B) and time-(E) dependent fashion. Similarly, type III collagen also increased in a dose- (C, D) and time- (F) dependent fashion. Results shown represent mean±SEM of 3 independent experiments. One-way ANOVA was used to compare the differences among groups and *P<0.05 in comparison with baseline levels (0 ng CTGF or at 0 hour).