Table 2.
The percent coverage of primary (passage 0) adult rat hepatocytes (ARH) seeded onto various multilayer films, as determined by phase contrast micrographs taken at 1 and 3 days in culture. Lack of data implies negligible cell attachment. A rat collagen I (rCI) coated well (Biocoat) serves as the control.
| Type of Film | Film Composition | % Coverage Day 1 | % Coverage Day 3 |
|---|---|---|---|
| Synthetic | (PAH-PSS)5 | 20–30% | 20–30% |
| (PAH-PSS)5-rCI | 30–40% | 20–30% | |
| (PAH-PSS)4-PAH | --- | --- | |
| (PAH-PSS)4-PAH-rCI | 30–40% | 20–30% | |
| Polysaccharide-Polypeptide | (PLL-ALG)5-X | --- | --- |
| (PLL-ALG)5-X-rCI | 70–80% | 60–70% | |
| (PLL-ALG)4-PLL-X | --- | --- | |
| (PLL-ALG)4-PLL-X-rCI | 60–70% | 60–70% | |
| Polypeptide | (PLL-PGA)5-X | 40–50% | --- |
| (PLL-PGA)5-X-rCI | 60–70% | 40–50% | |
| (PLL-PGA)4-PLL-X | 40–50% | --- | |
| (PLL-PGA)4-PLL-X-rCI | 70–80% | 60–70% | |
| Control | rCI | 70–80% | 60–70% |