Figure 1. Generation of COX-2 transgenic mice.

The COX-2 transgene (complete human COX-2 cDNA cloned into the albumin promoter-driven vector) was microinjected into mouse zygotes to generate transgenic mice by established method. (A) Schematic representation of the human COX-2 transgene with albumin promoter. (B) Southern blot analysis of tail genomic DNA showing the presence of COX-2 transgene in transgenic (TG) but not in wild-type (WT) littermates. The DNA samples were digested with Bam H1; the blot was hybridized with [³²P]-labeled human COX-2 cDNA probe. (C) PCR analysis of tail genomic DNA showing the presence of COX-2 transgene in transgenic (TG) mice but not in wild-type (WT) littermates. (D) Western blot showing successful expression of COX-2 protein in the liver tissues from COX-2 transgenic mice. Equal amounts of the mouse liver tissue proteins from transgenic mice (TG) and wild type (WT) littermates were subjected to SDS-PAGE and Western blotting using anti-human COX-2 antibody.