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. 2003 Oct 31;100(23):13184–13189. doi: 10.1073/pnas.2235011100

Table 1. Kinetic data for the 10 active substrates of GalK variant Y371H.

Wild type
Y371H
Sugar substrate Km, mM kcat, min—1 kcat/Km, mM—1·min—1 Km, mM kcat, min—1 kcat/Km, M—1·min—1
graphic file with name pq2235011003.jpg
d-Galactose (14) 2.1 108 51.4 5.6 220 39.3
2-Deoxy-d-galactose (18) 3.6 30 8.3 4.7 200 42.6
d-GalNH2(19) 2.9 11.7 4.0 8.8 260 29.5
d-Talose (20) 2.9 45.5 15.7
3-Deoxy-d-galactose (21) 6.4 5.1 0.8 10.1 64.6 6.4
6-Deoxy-d-galactose (22) 4.9 2.9 0.59 8.0 101 12.6
6-Amino-d-galactose (23) 21.3 149 7.0
d-Galacturonic acid (24) 3.2 58 18.1
l-Altrose (16) 5.2 80 15.4
l-Glucose (25) 2.7 65 24.1

—, No conversion.