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. 2009 Feb 12;4(2):e4468. doi: 10.1371/journal.pone.0004468

Figure 6. Exposure of PrPC-cells to different concentrations of 3F4 induces endocytosis or cross-linking of PrP.

Figure 6

(A) Immunostaining of M17 and PrPC cells exposed to 1 µg/ml of 3F4 for 5 days shows a prominent reaction in vesicular structures in M17 and PrPC cells (panels 1 and 2). Coalesced vesicles simulating aggregated PrPC are evident near the Golgi region and in the cytosol of PrPC cells (panel 2). Untreated PrPC-cells reacted with 8H4-anti-mouse-FITC show a prominent reaction at the plasma membrane as expected (panel 3). (B) Reaction of M17 and PrPC cells exposed to 12 µg/ml of 3F4 for 4 hours with anti-mouse FITC shows cross-linking of PrP on the plasma membrane of M17 and PrPC cells (panels 1 and 2, arrow) and a slight increase of reactivity in vesicular structures in the latter (panel 2, arrow-head). Similar exposure of N2a-cells to 3F4 and PrPC-cells to anti-Thy1 antibody followed by immunoreaction with 8H4-anti-mouse-FITC shows plasma membrane and Golgi reaction of endogenous PrP in N2a cells (panel 3) and plasma membrane distribution of PrP in Thy-1 exposed cells (panel 4). (Mouse PrP expressed by N2a cells does not react with 3F4).