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. Author manuscript; available in PMC: 2010 Feb 1.
Published in final edited form as: Biol Psychiatry. 2008 Sep 24;65(3):198–203. doi: 10.1016/j.biopsych.2008.08.015

Figure 1. Chromatin immunoprecipitation (ChIP) in postmortem brain.

Figure 1

Schematic overview of ChIP in postmortem brain, starting with tissue homogenization, collection and purification of nuclei, followed by the optional step of immunotagging and fluorescence-activated sorting of nuclei (into neuronal and non-neuronal, for example), followed by nuclei lysis and enzymatic digestion of chromatin fibers and polynucleosomes) into mono-nucleosomes, followed by immunoprecipitation with site- and modification-specific anti-histone antibodies (anti-tri-methyl-H3-lysine 4, for example), followed by separation of DNA and histones, and quantification of specific DNA sequences in the immunoprecipitate, relative to input. See (38, 55, 58) for details.