Effect of diet and exercise on caveolin-1 binding to endothelial nitric oxide synthase (eNOS) in the penis. (A) Representative Western immunoblot of caveolin-1 and eNOS in samples purified for NOSs in the penes of NFSe (line 1), NFEx (line 2), HFSe (line 3), and HFEx (line 4) pigs. (B) Quantitative analysis of caveolin-1 binding to eNOS in the penes of NFSe, NFEx, HFSe, and HFEx pigs. Binding of eNOS to caveolin-1 was increased in HFSe pig penes compared to NFSe pig penes, and this was prevented by exercise. Each bar represents the standard error of the mean (SEM). a = P < 0.05 vs. NFSe; N = 5; NF = normal-fat diet; HF = high-fat diet; Se = sedentary; Ex = exercised.