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. Author manuscript; available in PMC: 2009 Feb 9.
Published in final edited form as: Arch Biochem Biophys. 2007 Jan 12;459(1):1–9. doi: 10.1016/j.abb.2006.12.024

Figure 2.

Figure 2

Effect of redox potential shifting on PFL cleavage. The potential of the growth cultures were adjusted to the indicated values by air purge prior to lysis and exposure to air. Lanes 1–4: cultures overexpressing PFL. Lane 5: Cells overexpressing PFL exposed to air after N2 gas purge (without redox potential adjustment). Lane 6–9: PFL cells (at anaerobic potential of −68 mV) were lysed with AdhE cells at the indicated potentials. In all cases complete cleavage of PFL is observed.