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. 2008 Dec 12;417(Pt 1):183–193. doi: 10.1042/BJ20080868

Figure 8. Effect of HFD on 3-NT levels in liver mitochondrial proteins.

Figure 8

(A) Representative Western blots depicting the extent of nitration in mitochondrial proteins from mice fed either a control or HFD for 16 weeks. Representative Western blots for two separate pairs of mice are shown along with their duplicate stained total protein gels. (B) Quantification of 3-NT levels in mitochondrial proteins. Levels of nitrated proteins were normalized to total protein. (C) Quantification of total protein was performed on duplicate gels, stained with SyproRuby protein stain, used for 3-NT Western blots. Note there were no differences in total protein between control and HFD groups. Values represent the means±S.E.M. for three pairs of mice. **P<0.01, compared with control.