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Quantitation of nuclear c-Abl. Exponentially growing 10T½ cells were stably attached (Att), detached and kept in suspension for 60 min (Det), or plated on FN (A) or poly(l-lysine) (PL) (B). At the indicated times, cells were harvested and fractionated, and the nuclear fraction was examined for c-Abl protein by immunoblot analysis. Nuclear c-Abl in stably adherent cells was set to 100%. The values shown are mean and standard deviations from three fractionation experiments.
