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Plating on FN activates c-Abl tyrosine kinase. (A) 10T½ cells kept in suspension (time 0) or plated on FN or polylysine were extracted, c-Abl was immunoprecipitated, and kinase activity was determined. (B) Abl−/− cells stably transfected with hemagglutinin-tagged c-Abl or control transfected cells were plated on FN and analyzed as in A. (C) c-Abl tyrosine kinase activity was assayed in 10T½ cells after plating on anti-α5-integrin IgG. (Inset) Fold stimulation relative to control.
