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. 2009 Feb 20;5(2):e1000387. doi: 10.1371/journal.pgen.1000387

Figure 3. The inhibition of PARG function induces ectopic CBs and relocation of PARP1 protein from chromatin into CBs.

Figure 3

(A, B) The salivary glands dissected from the Parg27.1 mutants expressing PARP1-DsRed (red) transgene. DNA was stained with Draq5 dye. Antibody against LSM11 protein (green) (A) and anti-Coilin (green) (B) were used to detect Cajal bodies. N – nucleolus. (C) Fluorescent in situ hybridization of U85 scaRNA. The salivary glands dissected from the Parg27.1 mutants expressing PARPe-EGFP transgene. U85 scaRNA probe (green) detects CBLP. Rabbit antibody against GFP protein (red) detects PARPe-EGFP. To visualize chromatin (blue), mouse anti-histone H1 antibody was used. Arrows indicate CBLP with strong colocalization of U85 scaRNA and PARP protein.