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. 2009 Feb 20;5(2):e1000387. doi: 10.1371/journal.pgen.1000387

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Kotova et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

The expression of UAS::PARG-EGFP transgene was induced in early second-instar larvae (A, B), or in early third-instar larvae (C), using hsp::Gal4 driver induction. Salivary glands were dissected and analyzed by confocal microscopy after 20 hrs following Gal4 induction (A, C) and after 30 hrs following Gal4 induction (B). PARG-EGFP protein is green; PARP1-DsRed is red, and DNA (Draq5) is blue. An arrow indicates colocalization of PARG-EGFP and PARP1-DsRed in the CB-like particle. D. The expression of recombinant catalytically inactive PARG^AA-EGFP protein in Parg^27.1 mutant animals does not rescue PARP1-DsRed protein localization to chromatin (after 30 hrs following Gal4 induction). E. Western blot hybridization was used to detect the accumulation of pADPr in Parg^27.1 mutant and Parg^27.1 mutant expressing PARG^AA-EGFP protein, but not in wild-type and Parg^27.1 mutant expressing PARG-EGFP protein.