Table 1. Respiratory and ATP hydrolytic activities of mitochondria.

	Respiration rate, %					ATPase SA, μmol Pi·min-1·mg-1
Condition	State 4	+Oligo.	State 3	+CCCP	RCR	—Oligo.	+Oligo.	% of inhibition
SDC22	12 ± 2	14 ± 2	51 ± 2	100	4.3	11.5 ± 1.1	0.6 ± 0.2	95
SDC22 + Dox	12 ± 2	12 ± 2	48 ± 3	100	3.9	11.2 ± 0.2	0.6 ± 0.1	95
SDC6	12 ± 4	17 ± 3	43 ± 5	100	3.6	11.3 ± 1.7	0.7 ± 0.4	95
SDC6 + Dox	89 ± 5	16 ± 3	N	100	N	7.7 ± 1	0.8 ± 0.2	90

Mitochondria were isolated from SDC22 and SDC6 grown in rich glycerol plus ethanol medium (YPEG) with or without doxycycline as described in the legend of Fig. 1. Additions were 0.3 mg/ml proteins, 4 mM NADH (state 4), 400 μM ADP (state 3), 6 μg/ml oligomycin (+Oligo.), and 3 μM + CCCP. Respiratory control ratio (RCR) is expressed as state 3 to state 4 respiration rates. N, not applicable. The results shown are representative of duplicate experiments for SDC22 and quadruplicate experiments for SDC6. Respiratory activities are expressed as percentages of the maximal respiration rates assayed with CCCP (2,289 ± 329 nmol O·min^-1·mg^-1 for SDC22, 2,734 ± 272 nmol O·min^-1·mg^-1 for SDC22 plus Dox, 1,582 ± 368 nmol O·min^-1·mg^-1 for SDC6, and 1,778 ± 418 nmol O·min^-1·mg^-1 for SDC6 plus Dox). SA, specific activity.