A, Concentration dependence of release of IL-1β from mononuclear cells by HKa. Mononuclear cells (2.0×106/mL) were incubated with HKa (0, 300, 600, and 900 nM), BK (600 nM) for 60 minutes at 37°C and processed as in Figure 1. B, Release of IL-1β from mononuclear cells incubated with recombinant kininogen domains and subdomains. Mononuclear cells (2.0×106/mL) were incubated with GST-D3, GST-E7P (K255-Q292), GST-D5, GST-(K420-D474), GST-(H475-S626), GST-D6 or GST, or HBSSA for 60 minutes at 37°C and processed as in Figure 1.